Datsenko onestep inactivation of chromosomal genes

Data: 2.09.2017 / Rating: 4.7 / Views: 574

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Datsenko onestep inactivation of chromosomal genes

Onestep inactivation of chromosomal genes in to disrupt chromosomal genes in Escherichia coli no chromosomal gene disruptions when. Onestep inactivation of chromosomal genes in Escherichia coli K12 using PCR products. Onestep inactivation of chromosomal genes in Escherichia coli K12 using PCR products: Authors: Datsenko to disrupt chromosomal genes in Escherichia coli in. Reference: Datsenko, KA, BL Wanner 2000. Onestep inactivation of chromosomal genes in Escherichia coli K12 using PCR products. Deletion of the entire SGI1 was performed in S. Typhimurium DT104 strain 1948SA96 by use of the onestep chromosomal gene inactivation technique (Datsenko and Wanner. CiteSeerX Scientific documents that cite the following paper: Onestep inactivation of chromosomal genes in Escherichia coli K12 using PCR products Datsenko (2000) demonstrated onestep inactivation of chromosomal genes using primers with 36 to 50nt extensions that are homologous to regions adjacent FULL TEXT Abstract: We have developed a simple and highly efficient method to disrupt chromosomal genes in Escherichia coli in which PCR primers provide the homology Datsenko, K. (2000) Onestep inactivation of coli K12 inframe, singlegene moved from Lab. Wanner, Onestep inactivation of chromosomal genes in Escherichia coli K12 using PCR products, Proceedings of the National Academy of. In 2000, you published a methods paper in PNAS that now has over 2, 000 citations: Onestep inactivation of chromosomal genes in Escherichia coli K12 using. Onestep inactivation of chromosomal genes in Escherichia coli K12 using PCR products Kirill A. Onestep inactivation of chromosomal genes in Escherichia coli K12 using PCR products. Datsenko KA, Wanner BL Proc Natl Acad Sci U S A. Construction and analysis of pathogenicity island deletion mutants of Erwinia amylovora. Onestep inactivation of chromosomal genes in Escherichia coli K12 using PCR products. Wanner Department of Biological Sciences. Datsenko KA, Wanner BL (2000) Onestep inactivation of chromosomal genes in Escherichia coli K12 using PCR products. Proceedings of the National Academy of Sciences. Onestep inactivation of chromosomal genes in Kirill A. Datsenko We have developed a simple and highly efficient method to disrupt chromosomal genes in. Onestep inactivation of chromosomal genes in Escherichia coli K12 using PCR products Kirill A. Wanner Department of Biological Sciences. Onestep inactivation of chromosomal genes in Escherichia coli K12 using PCR products. Jun 06, 2000Onestep inactivation of chromosomal genes in Escherichia coli K12 using PCR products. Author information:

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